Electrochemical Lab-On-a-Chip


Several lab-on-a-chip (LOC) or µTAS platforms based on electrochemical detection schemes are being developed in the GTQ. Some of them are configured as micro-analytical systems for large number of consecutive measurements (e.g. in environmental monitoring). In relation to those systems, the main focus of the research is on the integration of solid state sensors, actuators, and microfluidic structures for a robust and fully automated functioning. Other LOC platforms are developed for the implementation of single use cartridges. In this case the focus is on conceiving new technologies that enable production at low-cost. Next are some examples of LOC platforms in development.

PMMA Systems based on comercial pumps and valves

Main characteristics:

  • Rapid prototyping with laser and CNC micromachining
  • Any type of electrochemical sensor can be integrated
  • Ideal for monitoring applications when energy, reagent or sample consumption are not critical
  • Easy to reconfigure for any particular application, including sample conditioning/preparation, calibration, cleaning
  • Simple replacement of sensors and active components 

Already applied to:

  • Analysis of heavy metals and pesticides in environmental water samples with gold amperometric sensors
  • Analysis of chemical oxygen demand in wastewater samples with an electrocatalytic composite-derived sensor

 

PMMA/PDMS SYSTEMS WITH INTEGRATED VALVES

 

  • Zero dead volume valves. High valve density
  • Low volume coupling of sensors to the microfluidics 
  • Ideal for monitoring applications requiring low consumption of sample and reagents (< 1µl per measurement/calibration cycle) and multiple measurement points (e.g. multiwell plates)

 

Already applied to: Cell culture monitoring

PDMS MICROFLUIDICS ON TRANSDUCER ARRAY CHIPS 

 

  • For measurement and cleaning automation
  • Channels embedded in the PDMS layers. No need for bonding between PDMS and transducer chip

 

Already applied to: Automation of a protein microarray readout process with an impedimetric transducer array 

 

WAX Microfluidics

 

  • Fully integrated systems including valves, pumps, reagent reservoirs, and electrodes for detection
  • Electrically controlled
  • Simple fabrication
  • Ideal for disposable cartridges

 

Already applied to: Sampler device for sequential acquisition of a large number of liquid or gaseous samples from the environment.

Capilarity driven systems with solid state sensors

Ultralow power systems for monitoring applications

In development

Projects

SWEATLOC: Lab-on-a-chip device for sweat glucose, lactate, ph and sweating rate monitoring
- Program: TECNIOSPRING - Ref:
Sun, 2015-03-01 00:00 - Tue, 2017-02-28 00:00
129.280€
129.280€
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ITACA: Investigación de tecnologías de tratamiento, reutilización y control para la sostenibilidad futura de la depuración de aguas residuales.
- Program: Contrato INNPRONTA - Ref:
Sat, 2011-10-01 00:00 - Wed, 2014-12-31 00:00
479.059€
143.000€
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DRUGLOC: Desarrollo de una plataforma Lab-on-a-Chip basada en actuadores de cera para la detección de drogas
- Program: Proyectos I+D+i - Retos 2013 - Ref:
Wed, 2014-01-01 00:00 - Sat, 2016-12-31 00:00
133.100€
133.100€
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Articles

Giménez-Gómeza, P; Gutiérrez-Capitán, M; Capdevila, F; Puig-Pujol, A; Fernández-Sánchez, C; Jiménez-Jorquera, C
"Robust l-malate bienzymatic biosensor to enable the on-site monitoring of malolactic fermentation of red wines"
Analytica Chimica Acta VL.954 (), 105-113, 2017
4.51
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Niu, PF; Asturias-Arribas, L; Gich, M; Fernandez-Sanchez, C; Roig, A
"Electrochemically Active Thin Carbon Films with Enhanced Adhesion to Silicon Substrates"
ACS APPLIED MATERIALS & INTERFACES VL.8 (45), 31092-31099, 2016
7.15
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Gimenez-Gomez, P; Gutierrez-Capitan, M; Capdevila, F; Puig-Pujol, A; Fernandez-Sanchez, C; Jimenez-Jorquera, C
"Monitoring of malolactic fermentation in wine using an electrochemical bienzymatic biosensor for L-lactate with long term stability"
ANALYTICA CHIMICA ACTA VL.905 (), 126-133, 2016
4.51
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Diaz-Gonalez, M; Fernandez-Sanchez, C; Baldi, A
"Multiple actuation microvalves in wax microfluidics"
LAB ON A CHIP VL.16 (20), 3969-3976, 2016
5.59
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Niu, PF; Fernandez-Sanchez, C; Gich, M; Navarro-Hernandez, C; Fanjul-Bolado, P; Roig, A
"Screen-printed electrodes made of a bismuth nanoparticle porous carbon nanocomposite applied to the determination of heavy metal ions"
MICROCHIMICA ACTA VL.183 (2), 617-623, 2016
3.74
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Alayo, N; Fernandez-Sanchez, C; Baldi, A; Esquivel, JP; Borrise, X; Perez-Murano, F
"Gold interdigitated nanoelectrodes as a sensitive analytical tool for selective detection of electroactive species via redox cycling"
MICROCHIMICA ACTA VL.183 (5), 1633-1639, 2016
4.83
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Diaz-Gonzalez, M; Gutierrez-Capitan, M; Niu, PF; Baldi, A; Jimenez-Jorquera, C; Fernandez-Sanchez, C
"Electrochemical devices for the detection of priority pollutants listed in the EU water framework directive"
TRAC-TRENDS IN ANALYTICAL CHEMISTRY VL.77 (), 186-202, 2016
6.47
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Gutierrez-Capitan, M; Baldi, A; Gomez, R; Garcia, V; Jimenez-Jorquera, C; Fernandez-Sanchez, C
"Electrochemical Nanocomposite-Derived Sensor for the Analysis of Chemical Oxygen Demand in Urban Wastewaters"
VL.87 (4), 2152-2160, 2015
5.64
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Diaz-Gonzalez, M; Salvador, JP; Bonilla, D; Marco, MP; Fernandez-Sanchez, C; Baldi, A
"A microfluidic device for the automated electrical readout of low-density glass-slide microarrays"
BIOSENSORS & BIOELECTRONICS VL.74 (), 698-704, 2015
6.41
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Gimenez-Gomez, P; Escude-Pujol, R; Jimenez-Jorquera, C; Gutierrez-Capitan, M
"Multisensor Portable Meter for Environmental Applications"
IEEE SENSORS JOURNAL VL.15 (11), 6517-6523, 2015
1.76
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Gutiérrez-Capitán,M; Ipatov, Andrey; Merlos,A; Jiménez-Jorquera, C; César Fernández-Sánchez
"Compact Electrochemical Flow System for the Analysis of Environmental Pollutants"
Electroanalysis VL.26 (1), 497-506, 2014
2.82
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Diaz-Gonzalez, M; Baldi, A
"Fabrication of Biofunctionalized Microfluidic Structures by Low-Temperature Wax Bonding"
Analytical chemistry VL.84 (18), 7838-7844, 2012
5.70
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Burdallo, I; Jimenez-Jorquera, C; Fernández-Sánchez, C; Baldi, A
"Integration of microelectronic chips in microfluidic systems on printed circuit board"
JOURNAL OF MICROMECHANICS AND MICROENGINEERING VL.22 (10), 105022-, 2012
2.11
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Patents

Dispositivo de lectura de microarrays de tipo eléctrico reutilizable
Applicant(s): CONSEJO SUPERIOR INVESTIGACION [ES] + (CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC))
Inventor(s: BALDI COLL ANTONIO [ES]; FERNANDEZ SANCHEZ CESAR [ES]; DE LA RICA QUESADA ROBERTO [ES]; BONILLA AGUILAR DIANA LISSETTE [CO]
Application number: ES20080002068 Fri, 2008-07-11 10:00
Priority number(s): ES20080002068 Fri, 2008-07-11 10:00
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Thesis

Sistema de lectura eléctrica de microarrays proteómicos
Diana Bonilla Agular
César Fernández-Sánchez; Antonio Baldi Coll
UAB
Thu, 2013-12-19 (All day)
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Integración de microsensores y estructuras microfluídicas para la medida de parámetros químicos en sistemas biológicos
Isabel Burdallo Bautista
Cecilia Jiménez-Jorquera; Antonio Baldi Coll
UAB
Thu, 2015-01-29 (All day)
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Carbon Xerogel Nanocomposite Materials for Electrochemical Devices: Application to Heavy Metal Detection
Pengfei Niu
César Fernández-Sánchez; Martí Gich
UAB
Fri, 2015-09-18 (All day)
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Dispositius impedimètrics per a la detecció directa de microorganismes
Maria Mallén
César Fernández-Sánchez; Antonio Baldi Coll
UAB
Fri, 2016-09-02 (All day)
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