Photonic Lab-On-a-Chip


Lab on a chip concept relies on the integration of the different steps of an analytical protocol (sample injection, filtering, processing, detection and neutralization) at the microscale. The great benefit of physically scaling down analytical systems is not only a reduction in footprint but rather an improvement of the analytical performance. In addition, with the synergic combination of photonics with lab-on-a-chip technologies, the photonic-lab-on-a-chip (PhLoC, also optofluidics or Photonic LOC) concept can be coined. Such a configuration has additional advantages as compared to its counterparts, as could be immunity to electromagnetic interferences or measurement in bulk. When related to screening of cell cultures or, more generally, to the measurement of smples of biological origin, photonics represents a huge advantage, since it allows to perform measurements without direct contact with the sample, or the use of electric potentials, which could either interfere or modify the cell properties. Nowadays, two different approaches towards PhLoC can be found in the literature: performance enhancement, which means the development of high-throughput systems (HTS) for multiparametric detection; or accuracy enhancement, reaching single cell analysis and manipulation.

Applications

 

  • Detection and quantification of minute concentration of aetiological agents, either in fluorescence or in absorbance
  • Cell counting by scattering
  • Particle counting and sizing by scattering
  • Cell screening (using the appropriate staining)
  • Label-free detection of pollutants, DNA strands, etc, by interferometry
  • Label-free detection of of pollutants, DNA strands, etc, by Raman spectroscopy on a chip
  • Acquisition of the photonic fingerprint (PIN) of complex samples (wine, oil, cell cultures, etc)

 

PhLoC consisting of a multiple internal reflection photonic
cuvette and a xerogel-based solid  state light emitter.

PhLoC comprising a biofunctionalized Tesla mixer monolithically integrated
with a multiple internal reflection photonic cuvette and an electrochemical cell. 

1x4 Liquid-liquid optofluidic router