Photonic Lab-On-a-Chip


Lab on a chip concept relies on the integration of the different steps of an analytical protocol (sample injection, filtering, processing, detection and neutralization) at the microscale. The great benefit of physically scaling down analytical systems is not only a reduction in footprint but rather an improvement of the analytical performance. In addition, with the synergic combination of photonics with lab-on-a-chip technologies, the photonic-lab-on-a-chip (PhLoC, also optofluidics or Photonic LOC) concept can be coined. Such a configuration has additional advantages as compared to its counterparts, as could be immunity to electromagnetic interferences or measurement in bulk. When related to screening of cell cultures or, more generally, to the measurement of smples of biological origin, photonics represents a huge advantage, since it allows to perform measurements without direct contact with the sample, or the use of electric potentials, which could either interfere or modify the cell properties. Nowadays, two different approaches towards PhLoC can be found in the literature: performance enhancement, which means the development of high-throughput systems (HTS) for multiparametric detection; or accuracy enhancement, reaching single cell analysis and manipulation.

Applications

 

  • Detection and quantification of minute concentration of aetiological agents, either in fluorescence or in absorbance
  • Cell counting by scattering
  • Particle counting and sizing by scattering
  • Cell screening (using the appropriate staining)
  • Label-free detection of pollutants, DNA strands, etc, by interferometry
  • Label-free detection of of pollutants, DNA strands, etc, by Raman spectroscopy on a chip
  • Acquisition of the photonic fingerprint (PIN) of complex samples (wine, oil, cell cultures, etc)

 

PhLoC consisting of a multiple internal reflection photonic
cuvette and a xerogel-based solid  state light emitter.

PhLoC comprising a biofunctionalized Tesla mixer monolithically integrated
with a multiple internal reflection photonic cuvette and an electrochemical cell. 

1x4 Liquid-liquid optofluidic router

Projects

HIP-LAB: High-throughput integrated photonic lab-on-a-DVD platforms
- Program: Support for frontier research (ERC)-ERC Starting Grant - Ref:
Wed, 2008-10-01 10:00 - Mon, 2013-09-30 10:00
1.717.200€
1.717.200€
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LIPHOS: Living Photonics: Monitoring light propagation through cells
- Program: FP7 - Ref:
Thu, 2012-11-01 00:00 - Sat, 2015-10-31 00:00
3.200.000€
631.473€
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Articles

Rodriguez-Ruiz, I; Ackermann, TN; Munoz-Berbel, X; Llobera, A
"Photonic Lab-on-a-Chip: Integration of Optical Spectroscopy in Microfluidic Systems"
ANALYTICAL CHEMISTRY VL.88 (13), 6630-6637, 2016
5.89
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Conejero-Muriel, M; Rodriguez-Ruiz, I; Verdugo-Escamilla, C; Llobera, A
"Continuous Sensing Photonic Lab-on-a-Chip Platform Based on Cross-Linked Enzyme Crystals"
ANALYTICAL CHEMISTRY VL.88 (23), 11919-11923, 2016
5.89
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Ackermann, TN; Gimenez-Gomez, P; Munoz-Berbel, X; Llobera, A
"Plug and measure - a chip-to-world interface for photonic lab-on-a-chip applications"
LAB ON A CHIP VL.16 (17), 3220-3226, 2016
5.59
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Pujol-Vila, F; Gimenez-Gomez, P; Santamaria, N; Antunez, B; Vigues, N; Diaz-Gonzalez, M; Jimenez-Jorquera, C; Mas, J; Sacristan, J; Munoz-Berbel, X
"Portable and miniaturized optofluidic analysis system with ambient light correction for fast in situ determination of environmental pollution"
SENSORS AND ACTUATORS B-CHEMICAL VL.222 (), 55-62, 2016
4.10
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Perdigones, F; Aracil, C; Quero, JM; Gutiérrez-Capitán, M; Jiménez-Jorquera, C; Giménez-Gómez, P
"Integration method of silicon sensors on SU‑8‑based microfluidic platforms"
Microsystem Technologies VL.21 (), 155-161, 2015
0.88
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Muller, P; Kopp, D; Llobera, A; Zappe, H
"Optofluidic router based on tunable liquid-liquid mirrors"
LAB ON A CHIP VL.14 (4), 737-743, 2014
5.70
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Ibarlucea, B; Diez-Gil, C; Ratera, I; Veciana, J; Caballero, A; Zapata, F; Tarraga, A; Molina, P; Demming, S; Buttgenbach, S; Fernandez-Sanchez, C; Llobera, A
"PDMS based photonic lab-on-a-chip for the selective optical detection of heavy metal ions"
ANALYST VL.138 (3), 839-844, 2013
4.23
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Rodriguez-Ruiz, I; Llobera, A; Vila-Planas, J; Johnson, DW; Gomez-Morales, J; Garcia-Ruiz, JM
"Analysis of the Structural integrity of SU-8-Based Optofluidic Systems for Small-Molecule Crystallization Studies"
ANALYTICAL CHEMISTRY VL.85 (20), 9678-9685, 2013
5.70
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Munoz-Berbel, X; Rodriguez-Rodriguez, R; Vigues, N; Demming, S; Mas, J; Buttgenbach, S; Verpoorte, E; Ortiz, P; Llobera, A
"Monolithically integrated biophotonic lab-on-a-chip for cell culture and simultaneous pH monitoring"
LAB ON A CHIP VL.13 (21), 4239-4247, 2013
5.70
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Ordeig, O; Ortiz, Pedro; Muñoz-Berbel, X; Demming, S; Büttgenbach, S; Fernández-Sánchez, C; Llobera, A
"Dual Photonic-Electrochemical Lab on a Chip for Online Simultaneous Absorbance and Amperometric Measurements"
Analytical Chemistry VL.84 (8), 3546-3553, 2012
5.87
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Vila-Planas, J; Fernandez-Rosas, E; Ibarlucea, B ; Demming, S; Nogues, C; Plaza, JA; Dominguez, C ; Buttgenbach, S; Llobera, A
"Cell analysis using a multiple internal reflection photonic lab-on-a-chip"
NATURE PROTOCOLS VL.6 (10), 1642-1655, 2011
8.36
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Ibarlucea, Bergoi; Fernandez-Rosas, Elisabet; Vila-Planas, Jordi; Demming, Stefanie; Nogues, Carme; Plaza, Jose A.; Buettgenbach, Stephanus; Llobera, Andreu
"Cell Screening Using Disposable Photonic Lab on a Chip Systems"
ANALYTICAL CHEMISTRY VL.82 (10), 4246-4251, 2010
5.21
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Llobera, A.; Cadarso, V.J.; Darder, M.; Dominguez, C.; Fernández-Sánchez, C.
"Full-field photonic biosensors based on tunable bio-doped sol-gel glasses"
LAB ON A CHIP VL.8 (7), 1185-1190, 2008
6.48
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Llobera, A.; Demming, S.; Wilke, R.; Buttgenbach, S.
"Multiple internal reflection poly(dimethylsiloxane) systems for optical sensing"
LAB ON A CHIP VL.7 (), 1560-1566, 2007
5.07
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Llobera, A.; Wilke, R.; Buttgenbach, S.
"Poly(dimethylsiloxane) hollow Abbe prism with microlenses for detection based on absorption and refractive index shift"
LAB ON A CHIP VL.4 (1), 24-27, 2004
5.05
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Llobera, A.; Wilke, R.; Buttgenbach, S.
"Poly(dimethylsiloxane) hollow Abbe prism with microlenses for detection based on absorption and refractive index shift"
LAB ON A CHIP VL.4 (1), 24-27, 2004
5.05
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Thesis

Photonic Devices based on hybrid organic-inorganic xerogel polymers
Ester Carregal Romero
Fernández, C; Llobera, A
Universitat Autònoma de Barcelona
Fri, 2012-11-16 (All day)
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Monolithically integrated polymeric Lab-on-(Bio)Chips with photonic/electrochemical detection
Bergoi Ibarlucea Cantón
César Fernández-Sánchez; Andreu Llobera Adan
UAB
Thu, 2013-11-14 (All day)
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PDMS-based optofluidic system
Jordi Vila Planas
Andreu LLobera; Xavier Muñoz-Berbel
UAB
Fri, 2014-06-20 (All day)
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MOEMS based on magnetic/photonic sensitive stimuli polymers
Sandra de Pedro
Andreu Llobera Adán, Victor Cadarso Busto
UAB
Fri, 2015-12-11 (All day)
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