Integrated opto-nanomechanical biosensor for functional genomic analysis
The aim of this project is to develop a portable biosensor microsystem able to detect hybridization of nucleic acids with sensitivity to distinguish single base substitutions, deletions and insertions. It will be mainly applied to studies of comparative expression analysis and identification of single nucleotide polymorphisms (SNPs) in human genes candidates to disease. The biosensor microsystem will consist of:
- an array of 20 microcantilevers
- an optical detection system of the cantilever motion,
- and a polymer microfluidic system.
The hybrid integration of the DNA biochip proposed, in which the array, detector and fluid transport are miniaturized and integrated into a single unit will be the first attempt in the integration of a nanobiodevice.