Poly(dimethylsiloxane) photonic microbioreactors based on segmented waveguides for local absorbance measurement


AU: 
Demming, S; Vila-Planas, J; Zadeh, SA; Edlich, A; Franco-Lara, E; Radespiel, R; Buttgenbach, S; Llobera, A
SO: 
ELECTROPHORESIS
VL: 
32
IS: 
3-4
BP: 
431
EP: 
439
PY: 
2011
SN: 
0173-0835
Impact: 
3.57
Year IF: 
2010
Abstract: 

We present the development of microbioreactors (MBRs) based on poly (dimethylsiloxane) (PDMS) segmented waveguides (SWG) for local absorbance measurements. Two different MBRs were studied, either using symmetric or asymmetric SWG (being defined as MBR-S and MBR-A, respectively). Their optical and fluidic performances were numerically analyzed, showing robustness from an optical point of view and distinct fluid flow profile. The optical characterization was done in two steps. Initially, the experimental limit of detection (LOD) and the sensitivity were determined for two different analytes (fluorescein and methylorange). With both systems, a similar limit of detection for both analytes was obtained, being in the micromolar level. Their sensitivities were 20.2 +/- 0.3 (x 10(-3)) A.U./mu M and 5.5 +/- 0.2 (x 10(-3)) A.U./mu M for fluorescein and methylorange, respectively. Once validated its applicability for local absorbance measurements, a continuous cultivation of Saccharomyces cerevisiae was done to test the viability of the proposed systems for photonic MBRs. Concretely, the cell growth was locally monitored inside the MBR during 33 h. Spectral analysis showed that the determination of the culture parameters were wavelength dependant, with a growth rate of 0.39 +/- 0.02 h(-1) and a doubling time of 1.65 +/- 0.09 h at an optimal wavelength of 469.9 +/- 0.3 nm. Besides the easy and monolithic integration of the SWG into poly(dimethylsiloxane) microfluidic systems, the results presented here are very promising for the application in any disposable photonic lab-on-a-chip systems used for online analysis or photonic MBRs.

DT: 
Article en revistes indexades
Percentil: 
HIGH
Percentil Year: 
2010
Category: 
CHEMISTRY, ANALYTICAL
KW: 
Absorbance measurements; Cell culture; Microbioreactors; Microfluidics; Photonic lab-on-a-chip