Electrodepositable alginate membranes for enzymatic sensors: An amperometric glucose biosensor for whole blood analysis


AU: 
Marquez, A; Jimenez-Jorquera, C; Dominguez, C; Munoz-Berbel, X
SO: 
BIOSENSORS & BIOELECTRONICS
GTQ Authors: 

Research Lines:

VL: 
97
BP: 
136
EP: 
142
PY: 
2017
SN: 
0956-5663
IF: 
7.78
Year IF: 
2016
Abstract: 

Simple and disposable point of care systems are usually the best solution for chronic patients to get a rapid diagnosis in home care context. However, their main drawback relies on the poor reliability derived from the low stability of the bio-recognition elements and low quality of the transducers. In the current work, we study the use of electrodeposited calcium alginate hydrogels as a biocompatible matrix in the development of enzymatic amperometric biosensors for whole blood analysis, to enhance the enzymes stability and to protect the transducer from biofouling. The alginate electrodeposition involves the controlled Ca2+ release, so the gel thickness can be modulated. In the biosensor, horseradish peroxidase (HRP) and glucose oxidase (GOD) were electrodeposited within the hydrogel and the activity of the bi-enzymatic system was analyzed chronoamperometrically using 3,3',5,5'-Tetramethylbenzidine (TMB) as the mediator. Besides enzyme entrapment, the obtained gels protected the transducer from biofouling, enabling the reuse of the transducer after hydrogel removal and re-electrodeposition. The biosensors showed good analytical characteristics to glucose determination in whole blood samples, discriminating among healthy and hyperglycemic samples, with good sensitivity (-0.27 mu A cm(-2) mM(-1)), low limit of detection (126 mu M) and long lineal range (2-12 mM).

DT: 
Article en revistes indexades
Percentil: 
HIGH
Percentil Year: 
2017
Category: 
CHEMISTRY, ANALYTICAL
KW: 
Alginate enzymatic membrane; Electrodeposited alginate; Glucose amperometric biosensor; Whole blood analysis; Biofouling