Cell analysis using a multiple internal reflection photonic lab-on-a-chip


AU: 
Vila-Planas, J; Fernandez-Rosas, E; Ibarlucea, B ; Demming, S; Nogues, C; Plaza, JA; Dominguez, C ; Buttgenbach, S; Llobera, A
SO: 
NATURE PROTOCOLS
Research Lines: 
Photonic Lab-On-a-Chip
VL: 
6
IS: 
10
BP: 
1642
EP: 
1655
PY: 
2011
SN: 
1754-2189
IF: 
8.36
Year IF: 
2010
Abstract: 

Here we present a protocol for analyzing cell cultures using a photonic lab-on-a-chip (PhLoC). By using a broadband light source and a spectrometer, the spectrum of a given cell culture with an arbitrary population is acquired. The PhLoC can work in three different regimes: light scattering (using label-free cells), light scattering plus absorption (using stained cells) and, by subtraction of the two former regimes, absorption (without the scattering band). The acquisition time of the PhLoC is similar to 30 ms. Hence, it can be used for rapid cell counting, dead/live ratio estimation or multiparametric measurements through the use of different dyes. The PhLoC, including microlenses, micromirrors and microfluidics, is simply fabricated in a single-mask process (by soft lithographic methods) using low-cost materials. Because of its low cost it can easily be implemented for point-of-care applications. From raw substrates to final results, this protocol can be completed in 29 h.

DT: 
Article en revistes indexades
Percentil: 
HIGH
Percentil Year: 
2010
Category: 
BIOCHEMICAL RESEARCH METHODS